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Column: Focus on Biopharmaceutical Analysis

Jun 01, 2019
LCGC North America
Coupling CE with MS presents some challenges. Here, we discuss the advantages of CE–MS over LC–MS, and the parameters that are important to obtain a stable CE–MS profile.
Jan 01, 2019
LCGC North America
Determining the higher order structure of a protein pharmaceutical is important. Here, we review the approaches for HOS determination that are currently receiving the most attention in the literature and at scientific meetings.
Nov 01, 2018
LCGC North America
The analytical techniques used for characterizing biotherapeutics have evolved. We review the utility of the traditional tools and discuss the new, orthogonal techniques that are increasingly being used.
Jun 01, 2018
LCGC North America
To ensure the reliable and accurate characterization of biotherapeutics, an arsenal of orthogonal analytical techniques is needed.
Jan 01, 2018
LCGC North America
A look at techniques for charge-variant analysis of monoclonal antibodies and the question of whether pH gradients are really better than salt gradients
Dec 01, 2017
LCGC North America
Several key applications of biolayer interferometry in pharmaceutical development have emerged recently. Here, we evaluate its use for ensuring product titer from fermentation, and compare the strengths and weaknesses of the technique to those of HPLC.
Jun 01, 2017
LCGC North America
Significant recent advances now enable routine usage of HDX-MS for comparing the conformations of biopharmaceutical products.
Jan 01, 2017
LCGC North America
What are the best methodologies for demonstrating comparability?
Sep 01, 2016
LCGC North America
For testing various attributes of proteins, CE–MS is superior to LC–MS. And despite common fears, it is a robust tool. But adapting an LC–MS method to CE–MS requires careful optimization.
Jul 01, 2016
LCGC North America
In this column, we introduce the basics of today’s approaches for doing intact protein dissociation with mass spectrometry (MS), or top-down sequencing (that is, rather than the more conventional peptide-based “bottom-up” sequencing where future improvements might occur, advantages and limitations of using top-down sequencing, possible applications, and why it has become such an important and pursued research area for many.
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